Laboratorio de Glicosistemas

Instituto de Investigaciones Químicas, CSIC – Universidad de Sevilla

Research lines

The main goal of the Glycosystems Laboratory is the study in detail of the biologically relevant recognition processes involving carbohydrates, with the aim to intervene on them. In particular, through different funded research projects, this laboratory is focused on the synthesis of carbohydrates, glycoconjugates, and carbohydrate multivalent systems, the study of these entities at the structural level using computational and Nuclear Magnetic Resonance (NMR) techniques, and the analysis of the interactions of these carbohydrate systems with their corresponding receptors using different approaches. The final goal of these studies is the understanding of these processes and the development of useful tools to be applied on glycobiology and biomedicine.

        1.- Synthesis of Carbohydrate Multivalent Systems. Applications as pathogen infection inhibitors and in the modulation of the immune response.

        2.- Synthesis of Glycodendripeptides as synthetic vaccines against allergy.

        3.- Synthesis of Glycosaminoglycans (GAGs) and Molecular Recognition studies by Protein Receptors.

    4.- 3D Structure of Carbohydrates and their Complexes with Receptor Proteins: Nuclear Magnetic Resonance and Molecular Modelling.

Researchers

F. Javier Rojo Marcos                          Noelia de la Cruz Ruiz                      
Pedro M. Nieto Mesa                           Jorge Losada Méndez
José Luis de Paz Carrera                    Miryam Torres                                             
Ana Sousa Hervés                              Pedro Ramírez López

Fundings (last 5 years selection)

1.- Instituto de salud Carlos III. RETICS, Red de Investigación de Reacciones Adversas a Alérgenos y Fármacos. Asma, reacciones adversas y alérgicas (ARADyAL). RD016/0006/0011 (2017-2021)
2.- MINECO. Herramientas glicodendríticas en química biológica: estudios de interacción y aplicaciones biomédicas. CTQ2017-86265-P (2018-2020).
3.- Proyectos de Excelencia de la Junta de Andalucía Química Biológica de carbohidratos. Implicaciones del reconocimiento del receptor Langerina.Ref. FQM-1303 (2015-2019).
4.- EU ITN-PEOPLE. Selective Carbohydrate Immunomodulators targeting C-type Lectin Receptors on Antigen Presenting Cells (IMMUNOSHAPE). Ref EU H2020 64870 (2015-2018)
5.- MINECO. Estructuras glicodendríticas como herramientas en química biológica: aplicaciones como agentes anti-infectivos y en inmunoterapia a través de la interacción con lectinas y TLRs. CTQ2014-52328-P (2015-2018).

Publications (last selection 5 years)

1.- N. Varga, I. Sutkeviciute, R. Ribeiro-Viana, A. Berzi, R. Ramdasi, A. Daghetti, G. Vettoretti, A. Amara, M. Clerici, J. Rojo, F. Fieschi, A. Bernardi. A multivalent inhibitor of the DC-SIGN dependent uptake of HIV-1 and Dengue virus. Biomaterials 2014, 35, 4175-4184.

2.- Juan C. Muñoz-García, Eric Chabrol, Romain R Vives, Aline Thomas, Jose L. de Paz, Javier Rojo, Anne Imberty, Frank Fieschi, Pedro M Nieto, and Jesús Angulo. Langerin-Heparin Interaction: Two Binding Sites for Small and Large Ligands as revealed by a combination of NMR Spectroscopy and Cross-Linking Mapping Experiments. J. Am. Chem. Soc. 2015, 137, 4100-4110.

3.- Antonio Muñoz, David Sigwalt, Beatriz M. Illescas, Joanna Luczkowiak, Laura Rodríguez, Iwona Nierengarten, Michel Holler, Jean-Serge Remy, Kevin Buffet, Stéphane P. Vincent, Javier Rojo, Rafael Delgado, Jean-François Nierengarten, Nazario Martín. Synthesis of giant globular multivalent glycofullerenes as potent inhibitors in a model of Ebola virus infection. Nature Chem. 2016, 8, 50-57. 

4.-Beatriz M. Illescas, Javier Rojo, Rafael Delgado, Nazario Martín. Multivalent Glycosylated Nanostructures to Inhibit Ebola Virus Infection. J. Am. Chem. Soc., 2017, 139, 6018-6025

5.-Laura Rodríguez-Pérez, Javier Ramos-Soriano, Alfonso Pérez-Sánchez, Beatriz M. Illescas, Antonio Muñoz, Joanna Luczkowiak, Fátima Lasala, Javier Rojo, Rafael Delgado, and Nazario Martín. Nanocarbon-Based Glycoconjugates as Multivalent Inhibitors of Ebola Virus Infection.  J. Am. Chem. Soc., 2018, 140, 9891-9898,

International Collaborations

1.- Prof. Franck Fieschi, Instituto de Biología Estructural (IBS), Grenoble (Francia)

2.- Prof. Anna Bernardi, Universidad de Milán, (Italia)

3.- Prof. Salomé Pinho, Universidad de Oporto, (Portugal)

4.- Dr. Ludovic Landemarre, GlycoDiag, Orleans (Francia)

5.- Prof. Simone Visentini, Universidad Politécnica de Milán, (Italia)

Contact:  javier.rojo@iiq.csic.es

                  

   

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